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20 μg crossmab  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 20 μg crossmab
    SE-HPLC chromatogram of a Complex Therapeutic 2 + 1 bsAb. During SE-HPLC of the 2 + 1 <t>CrossMAb</t> after protein A purification, HMW by-products were observed at low abundancy, representing less than 1% of the total area under the curve (AUC). The hypothesized by-products are indicated on the peaks representing HMW variants. Indicated fractions were collected for further analysis.
    20 μg Crossmab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20+%CE%BCg+crossmab/pmc09980510-318-6-17?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    20 μg crossmab - by Bioz Stars, 2026-08
    90/100 stars

    Images

    1) Product Images from "Characterization of high-molecular weight by-products in the production of a trivalent bispecific 2+1 heterodimeric antibody"

    Article Title: Characterization of high-molecular weight by-products in the production of a trivalent bispecific 2+1 heterodimeric antibody

    Journal: mAbs

    doi: 10.1080/19420862.2023.2175312

    SE-HPLC chromatogram of a Complex Therapeutic 2 + 1 bsAb. During SE-HPLC of the 2 + 1 CrossMAb after protein A purification, HMW by-products were observed at low abundancy, representing less than 1% of the total area under the curve (AUC). The hypothesized by-products are indicated on the peaks representing HMW variants. Indicated fractions were collected for further analysis.
    Figure Legend Snippet: SE-HPLC chromatogram of a Complex Therapeutic 2 + 1 bsAb. During SE-HPLC of the 2 + 1 CrossMAb after protein A purification, HMW by-products were observed at low abundancy, representing less than 1% of the total area under the curve (AUC). The hypothesized by-products are indicated on the peaks representing HMW variants. Indicated fractions were collected for further analysis.

    Techniques Used: Purification

    Gene reporter assays measuring variant potency. Dose-response curves show the binding of CD3 Fabs to CD3e expressed on JURKAT cells, meant to simulate downstream signaling. The tetravalent control has the highest potency compared to the 2 + 1 CrossMAb control. The HMW1 fraction, thought to contain the tetravalent variant, shows a remarkably similar response as the product control. The dimer (HMW2 fraction) shows a substantially reduced potency.
    Figure Legend Snippet: Gene reporter assays measuring variant potency. Dose-response curves show the binding of CD3 Fabs to CD3e expressed on JURKAT cells, meant to simulate downstream signaling. The tetravalent control has the highest potency compared to the 2 + 1 CrossMAb control. The HMW1 fraction, thought to contain the tetravalent variant, shows a remarkably similar response as the product control. The dimer (HMW2 fraction) shows a substantially reduced potency.

    Techniques Used: Variant Assay, Binding Assay

    SEC-nMS Analysis of the 2 + 1 CrossMAb Product. The mass of the analyzed product is 197,037.9 Da (cyan diamond). An inset shows the charge deconvoluted spectrum from 6000 to 8500 m/z. Gains and losses of Hex(1) and GlcNAc(1) are annotated by blue and green bars. A small portion of dimer is observed, likely formed through non-covalent interactions during storage.
    Figure Legend Snippet: SEC-nMS Analysis of the 2 + 1 CrossMAb Product. The mass of the analyzed product is 197,037.9 Da (cyan diamond). An inset shows the charge deconvoluted spectrum from 6000 to 8500 m/z. Gains and losses of Hex(1) and GlcNAc(1) are annotated by blue and green bars. A small portion of dimer is observed, likely formed through non-covalent interactions during storage.

    Techniques Used:



    Similar Products

    20 μg crossmab  (Thermo Fisher)
    90
    Thermo Fisher 20 μg crossmab
    SE-HPLC chromatogram of a Complex Therapeutic 2 + 1 bsAb. During SE-HPLC of the 2 + 1 <t>CrossMAb</t> after protein A purification, HMW by-products were observed at low abundancy, representing less than 1% of the total area under the curve (AUC). The hypothesized by-products are indicated on the peaks representing HMW variants. Indicated fractions were collected for further analysis.
    20 μg Crossmab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/20+%CE%BCg+crossmab/pmc09980510-318-6-17?v=Thermo+Fisher
    Average 90 stars, based on 1 article reviews
    20 μg crossmab - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    SE-HPLC chromatogram of a Complex Therapeutic 2 + 1 bsAb. During SE-HPLC of the 2 + 1 CrossMAb after protein A purification, HMW by-products were observed at low abundancy, representing less than 1% of the total area under the curve (AUC). The hypothesized by-products are indicated on the peaks representing HMW variants. Indicated fractions were collected for further analysis.

    Journal: mAbs

    Article Title: Characterization of high-molecular weight by-products in the production of a trivalent bispecific 2+1 heterodimeric antibody

    doi: 10.1080/19420862.2023.2175312

    Figure Lengend Snippet: SE-HPLC chromatogram of a Complex Therapeutic 2 + 1 bsAb. During SE-HPLC of the 2 + 1 CrossMAb after protein A purification, HMW by-products were observed at low abundancy, representing less than 1% of the total area under the curve (AUC). The hypothesized by-products are indicated on the peaks representing HMW variants. Indicated fractions were collected for further analysis.

    Article Snippet: Sample injection amounts of 20 μg CrossMAb were used and data acquisition was controlled by Chromeleon software (Thermo Fisher Scientific).

    Techniques: Purification

    Gene reporter assays measuring variant potency. Dose-response curves show the binding of CD3 Fabs to CD3e expressed on JURKAT cells, meant to simulate downstream signaling. The tetravalent control has the highest potency compared to the 2 + 1 CrossMAb control. The HMW1 fraction, thought to contain the tetravalent variant, shows a remarkably similar response as the product control. The dimer (HMW2 fraction) shows a substantially reduced potency.

    Journal: mAbs

    Article Title: Characterization of high-molecular weight by-products in the production of a trivalent bispecific 2+1 heterodimeric antibody

    doi: 10.1080/19420862.2023.2175312

    Figure Lengend Snippet: Gene reporter assays measuring variant potency. Dose-response curves show the binding of CD3 Fabs to CD3e expressed on JURKAT cells, meant to simulate downstream signaling. The tetravalent control has the highest potency compared to the 2 + 1 CrossMAb control. The HMW1 fraction, thought to contain the tetravalent variant, shows a remarkably similar response as the product control. The dimer (HMW2 fraction) shows a substantially reduced potency.

    Article Snippet: Sample injection amounts of 20 μg CrossMAb were used and data acquisition was controlled by Chromeleon software (Thermo Fisher Scientific).

    Techniques: Variant Assay, Binding Assay

    SEC-nMS Analysis of the 2 + 1 CrossMAb Product. The mass of the analyzed product is 197,037.9 Da (cyan diamond). An inset shows the charge deconvoluted spectrum from 6000 to 8500 m/z. Gains and losses of Hex(1) and GlcNAc(1) are annotated by blue and green bars. A small portion of dimer is observed, likely formed through non-covalent interactions during storage.

    Journal: mAbs

    Article Title: Characterization of high-molecular weight by-products in the production of a trivalent bispecific 2+1 heterodimeric antibody

    doi: 10.1080/19420862.2023.2175312

    Figure Lengend Snippet: SEC-nMS Analysis of the 2 + 1 CrossMAb Product. The mass of the analyzed product is 197,037.9 Da (cyan diamond). An inset shows the charge deconvoluted spectrum from 6000 to 8500 m/z. Gains and losses of Hex(1) and GlcNAc(1) are annotated by blue and green bars. A small portion of dimer is observed, likely formed through non-covalent interactions during storage.

    Article Snippet: Sample injection amounts of 20 μg CrossMAb were used and data acquisition was controlled by Chromeleon software (Thermo Fisher Scientific).

    Techniques: